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KMID : 1140120100150040277
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Cancer Prevention Research
2010 Volume.15 No. 4 p.277 ~ p.284
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Involvement of Small GTPase RhoA for Microvilli Formation in Polarity T Cell and Non-polarity T Cell
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Oh Ji-Youn
Seo Hee-Ju
Lee Jae-Seol
Kim Hae-Young
Moon Kyoung-Mi
Choi Woo-Bong
Kim Byung-Woo
Lee Jong-Hwan
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Abstract
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The microvilli of circulating lymphocytes play an important role as specialized touching mediators including L-selectin and VLA-4 for transmigration of emigration from circulation to tissue during normal immunosurveillance and in response to inflammation. The microvilli change of circulatory T cells are controlled by the stimulation of chemokines or the interaction of other cells. In addition to this, T cell polarity is also important for cell biological function, effectively. Small GTPase RhoA controls cytoskeleton reorganization and ERM protein is a cross-linker between F-actin cytoskeleton and membrane proteins such as CD44, ICAM1-3. Above molecules is thought to be implicated to the control of cell polarity including microvilli formation. The present study was tried to understand some relation ship between polarity and microvilli. First of all, polarized T cells show that cell surface is smooth surface feature without microvilli, whereas non-polarized T cells display microvilli on the cell surface observed with SEM. Microvilli of non-polarized Jurkat T cell was disrupted in experiment to investigate the involvement of RhoA-ROCK signaling by using ROCK specific inhibitor, Y27632. Cells treated with bombesin, which is Rac1 activator, display surface feature without microvilli. When T cells touch BLDS that was derived from mouse lymph node microvilli was disappeared. In polarized EL4 T cells bearing over-expressing plasmid of PDZ domain constructed to increase active form of RhoA, polarity was disappeared, whereas microvilli was produced. Collectively, these data suggest that small GTPase RhoA and Rac1 is involved in microvilli formation.
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KEYWORD
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Microvilli, RhoA, ERM, Cell migration
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